Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000970)
SBP Name
Human VH dAb anti-CD16A VH E11
Synonyms
Human VH dAb E11
Molecular Weight 15 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli HB2151
Selection Method Phage display
Highest Status Research
SBP Sequence
>CDR1
GFTFSSYG
>CDR3
ARVGSFDF
Template Name Human VH3C23
Protein Scaffold Information of This SBP
Scaffold ID PS039
Scaffold Info
[1] , [2]
Scaffold Name Human VH dAb
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
CD16A
BTS Info
Activator Cancers [ICD-11: 2D4Z] EC50: 8 nM National Institutes of Health [1] , [2]
References
1 Identification of high-affinity anti-CD16A allotype-independent human antibody domains. Exp Mol Pathol. 2016 Oct;101(2):281-289.
2 Construction of a large phage-displayed human antibody domain library with a scaffold based on a newly identified highly soluble, stable heavy chain variable domain. J Mol Biol. 2008 Oct 10;382(3):779-89.