Details of the PSS | SYNBIP

General Information of Protein Scaffold of SBP (PSS) (ID: PS055)
PSS Name	Repebody
Synonyms	LRR-based binder
Diagrammatic Sketch
Highest Status	Research
Class	Non-Antibody
Fold Type	Alpha-Helices + Beta-Sheets + Loops
Thermal Denaturation TEMP	82℃
Sequence Length	170 + n * (20-29)
Molecular Weight	27-40 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym		PDB ID	UniProt ID	Origin
Leucine-rich repeat module of variable lymphocyte receptor	LRR protein; LRR of VLRs		3RFJ ; 3RFS ; 4J4L	A0A477B311	Jawless vertebrates: Listeria monocytogenes; Petromyzon marinus (Sea lamprey)

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
Repebody anti-Anaphylatoxin-C5a r-3E8	Research	Repebody	Escherichia coli Origami B (DE3)	Phage display	SBP Info	[1]
Repebody anti-Bcl-2 D3	Research	Repebody	Escherichia coli Origami B (DE3)	Phage display	SBP Info	[2]
Repebody anti-IgG-Fc D10	Research	Repebody	Escherichia coli	Phage display	SBP Info	[3]
Repebody anti-IgG-Fc E10	Research	Repebody	Escherichia coli	Phage display	SBP Info	[3]
Repebody anti-IgG-Fc F4	Research	Repebody	Escherichia coli	Phage display	SBP Info	[3]
Repebody anti-IL-6 B3	Research	Repebody	Escherichia coli	Phage display	SBP Info	[4]
Repebody anti-IL-6 C8	Research	Repebody	Escherichia coli	Phage display	SBP Info	[4]
Repebody anti-IL-6 F11	Research	Repebody	Escherichia coli	Phage display	SBP Info	[4]
Repebody anti-VEGF r-C2	Research	PDBID 3RFS	Escherichia coli Origami B (DE3)	Phage display	SBP Info	[5]
Repebody anti-VEGF r-F1	Research	PDBID 3RFS	Escherichia coli Origami B (DE3)	Phage display	SBP Info	[5]
Repebody anti-Btk rF10	Research	N.A.	Escherichia coli Origami (DE3)	Phage display	SBP Info	[6]
Repebody anti-ERBB1 rEgH9	Research	N.A.	N.A.	Phage display	SBP Info	[7]
Repebody anti-IL-6 r-D3E8	Research	N.A.	N.A.	Phage display	SBP Info	[8]
Repebody anti-IL-6 r-D3E8C4	Research	N.A.	N.A.	Phage display	SBP Info	[8]
Repebody anti-ERBB1 rAC1	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Repebody anti-ERBB1 rEgH9CCaaX	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Repebody anti-HEWL	Research	N.A.	N.A.	N.A.	SBP Info	[4]
Repebody anti-MD2	Research	N.A.	N.A.	N.A.	SBP Info	[4]

References

1	Effective suppression of C5a-induced proinflammatory response using anti-human C5a repebody. Biochem Biophys Res Commun. 2016 Sep 2;477(4):1072-1077.
2	Targeted delivery of a human Bcl-2-specific protein binder effectively induces apoptosis of cancer cells. Biochem Biophys Res Commun. 2020 May 28;526(2):447-452.
3	Protein binder for affinity purification of human immunoglobulin antibodies. Anal Chem. 2014 Jun 17;86(12):6019-25.
4	Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering. Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3299-304.
5	Anti-Human VEGF Repebody Effectively Suppresses Choroidal Neovascularization and Vascular Leakage. PLoS One. 2016 Mar 25;11(3):e0152522.
6	Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms. Nat Commun. 2020 May 8;11(1):2319.
7	Enzymatic prenylation and oxime ligation for the synthesis of stable and homogeneous protein-drug conjugates for targeted therapy. Angew Chem Int Ed Engl. 2015 Oct 5;54(41):12020-4. doi: 10.1002/anie.201505964. Epub 2015 Aug 28.
8	A high-affinity protein binder that blocks the IL-6/STAT3 signaling pathway effectively suppresses non-small cell lung cancer. Mol Ther. 2014 Jul;22(7):1254-1265.