Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000402)
SBP Name
Repebody anti-Anaphylatoxin-C5a r-3E8
Synonyms
Repebody r-3E8
Molecular Weight 29.0 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli Origami B (DE3)
Selection Method Phage display
Highest Status Research
PDB ID 5B4P
Sequence Length 261
SBP Sequence
>Repebody anti-Anaphylatoxin-C5a r-3E8
ALETITVSTPIKQIFPDDAFAETIKANLKKKSVTDAVTQNELNSIDQIIANNSDIKSVQG
IQYLPNVRYLALGGNKLHDISALKELTNLTYLFLDFNQLQLPNGVFDKLTNLKELYLSPN
QLQSLPDGVFDKLTNLTILGLDMNQLQSLPKGVFDKLTNLTELDLSYNQLQSLPKGVFDK
LTQLKDLSLSYNQLKSVPDGVFDRLTSLQYIWLHDNPWDCTCPGIRYLSEWINKHSGVVG
GPDSAKCSGSGKPVRSIICPT
3D Structure
Experimentally Validated Structure
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Template Name Repebody
Protein Scaffold Information of This SBP
Scaffold ID PS055
Scaffold Info
[1]
Scaffold Name Repebody
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Complement C5
BTS Info
Inhibitor C5a-involved inflammatory diseases Kd: 5 nM Korea Advanced Institute of Science and Technology (KAIST) [1]
References
1 Effective suppression of C5a-induced proinflammatory response using anti-human C5a repebody. Biochem Biophys Res Commun. 2016 Sep 2;477(4):1072-1077.