Details of the PSS | SYNBIP

General Information of Protein Scaffold of SBP (PSS) (ID: PS009)
PSS Name	Alpha-Rep protein
Synonyms	Alpha helicoidal repeat protein; Rep protein
Diagrammatic Sketch
Highest Status	Research
Class	Non-Antibody
Fold Type	Alpha-Helices + Loops
Thermal Denaturation TEMP	70-85℃
Sequence Length	157-253
Molecular Weight	17-37 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym		PDB ID	UniProt ID	Origin
HEAT-like repeat	N.A.		N.A.	N.A.	Synthetic construct

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
alphaRep protein anti-FNE clone 3	Research	Spy0125	Escherichia coli M15	Phage display	SBP Info	[1], [2]
alphaRep protein anti-Alpha-tubulin iE5	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[3]
alphaRep protein anti-Alpha-tubulin iiH5	Research	N.A.	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[3]
alphaRep protein anti-AlphaRep-A3 clone bA3-17	Research	N.A.	Escherichia coli M15	Phage display	SBP Info	[4]
alphaRep protein anti-AlphaRep-A3 clone bA3-2	Research	N.A.	Escherichia coli M15	Phage display	SBP Info	[5], [4]
alphaRep protein anti-AlphaRep-bA3-2 clone A3	Research	N.A.	Escherichia coli M15	Phage display	SBP Info	[5], [6]
alphaRep protein anti-Ebs1 clone 6	Research	N.A.	Escherichia coli M15	Phage display	SBP Info	[4]
alphaRep protein anti-GFP clone A	Research	N.A.	Escherichia coli	Phage display	SBP Info	[7]
alphaRep protein anti-GFP clone C	Research	N.A.	Escherichia coli	Phage display	SBP Info	[7]
alphaRep protein anti-GFP clone D	Research	N.A.	Escherichia coli	Phage display	SBP Info	[7]
alphaRep protein anti-GFP clone S6	Research	bGFP-D	N.A.	N.A.	SBP Info	[8]
alphaRep protein anti-GST-CA21-SP1-NC clone 9A8	Research	N.A.	Escherichia coli M15	N.A.	SBP Info	[9]
alphaRep protein anti-Alpha-tubulin iiiA5	Research	N.A.	N.A.	N.A.	SBP Info	[10]

References

1	The Rep artificial repeat protein scaffold: a new tool for crystallization and live cell applications. Biochem Soc Trans. 2015 Oct;43(5):819-24.
2	Structural and functional analysis of the fibronectin-binding protein FNE from Streptococcus equi spp. equi. FEBS J. 2014 Dec;281(24):5513-31.
3	Selection and Characterization of Artificial Proteins Targeting the Tubulin Subunit. Structure. 2019 Mar 5;27(3):497-506.e4.
4	Selection of specific protein binders for pre-defined targets from an optimized library of artificial helicoidal repeat proteins (alphaRep). PLoS One. 2013 Aug 27;8(8):e71512.
5	Ligand-induced conformational switch in an artificial bidomain protein scaffold. Sci Rep. 2019 Feb 4;9(1):1178.
6	alphaRep A3: A Versatile Artificial Scaffold for Metalloenzyme Design. Chemistry. 2017 Jul 26;23(42):10156-10166.
7	Specific GFP-binding artificial proteins (Rep): a new tool for in vitro to live cell applications. Biosci Rep. 2015 Jun 12;35(4):e00223.
8	Alpha repeat proteins (Rep) as expression and crystallization helpers. J Struct Biol. 2018 Feb;201(2):88-99.
9	Occupation of a thermoresistant-scaffold (Rep) at SP1-NC cleavage site disturbs the function of HIV-1 protease. Biosci Rep. 2020 Jun 26;40(6):BSR20201131.
10	Insight into microtubule nucleation from tubulin-capping proteins. Proc Natl Acad Sci U S A. 2019 May 14;116(20):9859-9864.