General Information of Synthetic Binding Protein (SBP) (ID: SBP000666)
SBP Name
alphaRep protein anti-Alpha-tubulin iiH5
Synonyms
alphaRep protein iiH5
Molecular Weight 17.3 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Phage display
Highest Status Research
PDB ID 6GWD
Sequence Length 157
SBP Sequence
>alphaRep protein anti-Alpha-tubulin iiH5
EKVEMYIKNLQDDSPPVRFNAAVALGKIGDERAVEPLIKALKDEDWQVRKTAAYALGKIG
DERAVEPLIKALKDEDRYVRSRAALALGKIGDERAVEPLIKALKDEDEYVRLSAASALGK
IGGERVRAAMEKLAETGTGFARKVAVNYLETHKSLIS
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS009
Scaffold Info
[1]
Scaffold Name Alpha-Rep protein
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Tubulin alpha-1A chain
BTS Info
Binder Research tool Kd: 95 nM Institute for Integrative Biology of the Cell (I2BC) [1]
References
1 Selection and Characterization of Artificial Proteins Targeting the Tubulin Subunit. Structure. 2019 Mar 5;27(3):497-506.e4.