General Information of Synthetic Binding Protein (SBP) (ID: SBP000662)
SBP Name
alphaRep protein anti-GFP clone D
Synonyms
alphaRep protein bGFP-D
Molecular Weight 20.8 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
Sequence Length 191
SBP Sequence
>alphaRep protein anti-GFP clone D
TDPEKVEMYIKNLQDDSMPVRYDAADALGKIGDERAVEPLIKALKDEDPNVRASAADALG
KIGDERAVEPLIKALKDEDGYVRFSAALALGKIGDERAVEPLIKALKDEDSRVRWSAAYA
LGQIGDERAVEPLIKALKDEDWQVRKAAAVALGKIGGERVRAAMEKLAETGTGFARKVAV
NYLETHKSLIS
3D Structure
Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS009
Scaffold Info
[1]
Scaffold Name Alpha-Rep protein
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Green fluorescent protein
BTS Info
Binder Tools for tracking, modulating or interfering with intracellular processes Kd: 14 nM Institute for Integrative Biology of the Cell (I2BC) [1]
References
1 Specific GFP-binding artificial proteins (Rep): a new tool for in vitro to live cell applications. Biosci Rep. 2015 Jun 12;35(4):e00223.