General Information of Synthetic Binding Protein (SBP) (ID: SBP000668)
SBP Name
alphaRep protein anti-AlphaRep-A3 clone bA3-17
Synonyms
alphaRep protein bA3-17
Thermal Denaturation TEMP 75.9 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli M15
Selection Method Phage display
Highest Status Research
SBP Sequence
>N-cap
W18, Q19, R22, V23, E26, K30
>Motif 1
L18, A19, R22, N23, L26, K30
>Motif 2
P18, F19, R22, V23, Q26, K30
Protein Scaffold Information of This SBP
Scaffold ID PS009
Scaffold Info
[1]
Scaffold Name Alpha-Rep protein
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
AlphaRep A3
BTS Info
Binder Tools as binders for pre-defined targets Kd: 141 nM Institute of integrated cell biology; Institute of molecular and cellular biochemistry (IBBMC) [1]
References
1 Selection of specific protein binders for pre-defined targets from an optimized library of artificial helicoidal repeat proteins (alphaRep). PLoS One. 2013 Aug 27;8(8):e71512.