General Information of Synthetic Binding Protein (SBP) (ID: SBP000663)
SBP Name
alphaRep protein anti-GFP clone S6
Synonyms
alphaRep protein bGFP-S6
Molecular Weight 20.5 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Highest Status Research
Sequence Length 191
SBP Sequence
>alphaRep protein anti-GFP clone S6
TDPEKVEMYIKNLQDDSGYVRSRAAEALGKIGDERAVEPLIKALKDEDPDVRKAAADALG
KIGDERAVEPLIKALKDEDANVRISAAQALGKIGDERAVEPLIKALKDEDPYVRIEAALA
LGKIGDERAVEPLIKALKDEDKAVRISAAAALGEIGGERVRAAMEKLAETGTGFARKVAV
NYLETHKSLIS
3D Structure
Computationally Modelled Structure
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Template Name bGFP-D
Protein Scaffold Information of This SBP
Scaffold ID PS009
Scaffold Info
[1]
Scaffold Name Alpha-Rep protein
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Green fluorescent protein
BTS Info
Binder Tools as expression and crystallization helpers Kd: 470 nM Institute for Integrative Biology of the Cell (I2BC) [1]
References
1 Alpha repeat proteins (Rep) as expression and crystallization helpers. J Struct Biol. 2018 Feb;201(2):88-99.