General Information of Synthetic Binding Protein (SBP) (ID: SBP000672)
SBP Name
alphaRep protein anti-Ebs1 clone 6
Synonyms
alphaRep protein bEbs1-6
Molecular Weight 31 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli M15
Selection Method Phage display
Highest Status Research
SBP Sequence
>N-cap
L18, L19, F22, D23, V26, K30
>Motif 1
W18, L19, F22, S23, W26, E30
>Motif 2
S18, A19, T22, A23, L26, K30
>Motif 3
A18, S19, E22, E23, R26, K30
>Motif 4
A18, A19, Y22, G23, A26, E30
>Motif 5
R18, Q19, S22, A23, D26, E30
>Motif 6
E18, F19, F22, S23, D26, E30
>Motif 7
A18, S19, F22, S23, E26, E30
Protein Scaffold Information of This SBP
Scaffold ID PS009
Scaffold Info
[1]
Scaffold Name Alpha-Rep protein
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Protein EBS1
BTS Info
Binder Tools as binders for pre-defined targets Kd: 12.3 nM Institute of molecular and cellular biochemistry and Biophysics; University of Southern Paris [1]
References
1 Selection of specific protein binders for pre-defined targets from an optimized library of artificial helicoidal repeat proteins (alphaRep). PLoS One. 2013 Aug 27;8(8):e71512.