General Information of Protein Scaffold of SBP (PSS) (ID: PS023)
PSS Name
Cyclotide
Synonyms
Head-to-tail cyclized cystine-knot; CCK
Diagrammatic Sketch
Highest Status Research
Class Non-Antibody
Fold Type Beta-Sheets + Loops
Thermal Denaturation TEMP >100℃
Sequence Length 28-54
Molecular Weight 3-6 kDa
Parental Protein of This PSS
Parental Protein Name Synonym PDB ID UniProt ID Origin
Kalata B1 Kalata-B1
1K48
P56254
Oldenlandia affinis
Momordica cochinchinensis trypsin inhibitor MCoTI-I/II
1IB9
P82409
Momordica cochinchinensis
Synthetic Binding Protein (SBP) Featuring this PSS
SBP Name Highest Status Template Expression System Selection Method Details Ref
Cyclotide anti-Abl MTAbl06 Research MCoTI-II N.A. Bacteria display
SBP Info
[1]
Cyclotide anti-Abl MTAbl07 Research MCoTI-II N.A. Bacteria display
SBP Info
[1]
Cyclotide anti-Abl MTAbl13 Research MCoTI-II N.A. Bacteria display
SBP Info
[1]
Cyclotide anti-CXCR4 MCo-CVX-5c Research MCoTI-I N.A. N.A.
SBP Info
[2]
Cyclotide anti-HMVEC MCo-TSP-1 Research SFTI-1 N.A. N.A.
SBP Info
[3]
Cyclotide anti-MAS1 MCo-AT1-7 Research MCoTI-II N.A. N.A.
SBP Info
[4]
Cyclotide anti-Mast cell tryptase beta MCoEETI(KKV) Research MCoEETI-II N.A. N.A.
SBP Info
[5]
Cyclotide anti-Mast cell tryptase beta MCoEETI(SDG) Research MCoEETI-II N.A. N.A.
SBP Info
[5]
Cyclotide anti-MDM2/HdmX MCo-PMI-6CIW Research MCoTI-I N.A. N.A.
SBP Info
[6]
Cyclotide anti-MDM2/HdmX MCo-PMI-K37R Research MCoTI-I N.A. N.A.
SBP Info
[6]
Cyclotide anti-NRP1/NRP2 N2.1C Research N.A. N.A. Bacteria display
SBP Info
[7]
Cyclotide anti-SET MCOG1 Research MCoTI-II N.A. N.A.
SBP Info
[8]
Cyclotide anti-SET MCOG2 Research MCoTI-II N.A. N.A.
SBP Info
[8]
Cyclotide anti-VEGF-A Research N.A. N.A. N.A.
SBP Info
[9]
References
1 Design of substrate-based BCR-ABL kinase inhibitors using the cyclotide scaffold. Sci Rep. 2015 Aug 12;5:12974.
2 Design of a novel cyclotide-based CXCR4 antagonist with anti-human immunodeficiency virus (HIV)-1 activity. J Med Chem. 2012 Dec 13;55(23):10729-34.
3 Cyclic thrombospondin-1 mimetics: grafting of a thrombospondin sequence into circular disulfide-rich frameworks to inhibit endothelial cell migration. Biosci Rep. 2015 Oct 13;35(6):e00270.
4 Design of a MCoTI-Based Cyclotide with Angiotensin (1-7)-Like Activity. Molecules. 2016 Jan 26;21(2):152.
5 Engineered cystine knot miniproteins as potent inhibitors of human mast cell tryptase beta. J Mol Biol. 2010 Jan 8;395(1):167-75.
6 In vivo activation of the p53 tumor suppressor pathway by an engineered cyclotide. J Am Chem Soc. 2013 Aug 7;135(31):11623-11633.
7 Design of a cyclotide antagonist of neuropilin-1 and -2 that potently inhibits endothelial cell migration. ACS Chem Biol. 2013;8(6):1147-54.
8 Using the MCoTI-II Cyclotide Scaffold To Design a Stable Cyclic Peptide Antagonist of SET, a Protein Overexpressed in Human Cancer. Biochemistry. 2016 Jan 19;55(2):396-405.
9 Engineering stabilized vascular endothelial growth factor-A antagonists: synthesis, structural characterization, and bioactivity of grafted analogues of cyclotides. J Med Chem. 2008 Dec 25;51(24):7697-704.