Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS006)
PSS Name	Affimer
Synonyms	Adhirons; Affimer proteins
Diagrammatic Sketch
Highest Status	Research
Class	Non-Antibody
Fold Type	One Alpha-Helix + Beta-Sheets+ Loops
Thermal Denaturation TEMP	70-101℃
Sequence Length	100-116
Molecular Weight	11-14 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym	PDB ID	UniProt ID	Origin
Cysteine proteinase inhibitor 1	Phytocystatin; KCPI1; Phytocystatin	N.A.	Q6TPK4	Actinidia deliciosa
Stefin A	Cystatin-A; Cystatin-AS	1DVC	P01040	Human

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
Affimer anti-Fibrinogen/Fibrin F5	Research	Adhiron	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[1]
Affimer anti-Fibrinogen/Fibrin G2	Research	Adhiron	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[1]
Affimer anti-ySUMO clone 10	Research	Adhiron92	Escherichia coli	Phage display	SBP Info	[2]
Affimer anti-ySUMO clone 15	Research	Adhiron92	Escherichia coli	Phage display	SBP Info	[2]
Affimer anti-ySUMO clone 20	Research	Adhiron92	Escherichia coli	Phage display	SBP Info	[2]
Affimer anti-ySUMO clone 22	Research	Adhiron92	Escherichia coli	Phage display	SBP Info	[2]
Affimer anti-HER4	Research	N.A.	Escherichia coli	Phage display	SBP Info	[3]
Affimer anti-HVT-UL49	Research	N.A.	Escherichia coli	Phage display	SBP Info	[3]
Affimer anti-K33-diubiquitin	Research	Adhiron	N.A.	Phage display	SBP Info	[4]
Affimer anti-K6-diubiquitin	Research	Adhiron	N.A.	Phage display	SBP Info	[4]
Affimer anti-p85	Research	N.A.	Escherichia coli	Phage display	SBP Info	[3]
Affimer anti-TNC	Research	N.A.	Escherichia coli	Phage display	SBP Info	[3]
Affimer anti-VEGFR-2 A9	Research	N.A.	Escherichia coli	Phage display	SBP Info	[3]
Affimer anti-VEGFR-2 B8	Research	N.A.	Escherichia coli	Phage display	SBP Info	[3]
Affimer MIA-1	Research	N.A.	Escherichia coli	Phage display	SBP Info	[5]
Affimer MIA-2	Research	N.A.	Escherichia coli	Phage display	SBP Info	[5]
Affimer anti-Fc-gamma-RIIIA F4	Research	N.A.	N.A.	Phage display	SBP Info	[6]
Affimer anti-Fc-gamma-RIIIA G3	Research	N.A.	N.A.	Phage display	SBP Info	[6]
Affimer anti-Alpha-2-AP AVA-016	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-CD27 AVA-014	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-CD3/CD22 AVA-012	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-CD3/CD3E AVA-002	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-CPMV clone 3	Research	N.A.	N.A.	N.A.	SBP Info	[8]
Affimer anti-GITR AVA-018	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-LAG-3 AVA-017	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-PD-L1 AVA-004	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-PD-L1/LAG-3 AVA-021	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-SARS-CoV-2	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-TPBG AVA-020	Research	N.A.	N.A.	N.A.	SBP Info	[7]
Affimer anti-Trastuzumab AVA00106	Research	N.A.	N.A.	N.A.	SBP Info	[7]

References

1	Affimer proteins as a tool to modulate fibrinolysis, stabilize the blood clot, and reduce bleeding complications. Blood. 2019 Mar 14;133(11):1233-1244.
2	Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein Eng Des Sel. 2014 May;27(5):145-55.
3	Affimer proteins are versatile and renewable affinity reagents. Elife. 2017 Jun 27;6:e24903.
4	Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling. Mol Cell. 2017 Oct 5;68(1):233-246.e5.
5	Phage display selected magnetite interacting Adhirons for shape controlled nanoparticle synthesis. Chem Sci. 2015 Oct 1;6(10):5586-5594.
6	Affimer proteins inhibit immune complex binding to FcRIIIa with high specificity through competitive and allosteric modes of action. Proc Natl Acad Sci USA. 2018 Jan 2;115(1):E72-E81.
7	Avacta. Product Development Pipeline. 2021.
8	Affimer reagents as tools in diagnosing plant virus diseases. Sci Rep. 2019 May 17;9(1):7524.