General Information of Synthetic Binding Protein (SBP) (ID: SBP000354)
SBP Name
Affimer anti-ySUMO clone 15
Synonyms
Ad-ySUMO 15
Molecular Weight 12.0 kDa
Thermal Denaturation TEMP 87.7 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
Sequence Length 103
SBP Sequence
>Affimer anti-ySUMO clone 15
ATGVRAVPGNENSLEIEELARFAVDEHNKKENALLEFVRVVKAKEQIDLTQPHDSTMYYL
TLEAKDGGKKKLYEAKVWVKYEEDEYWRMNFKELQEFKPVGDA
3D Structure
Computationally Modelled Structure
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Template Name Adhiron92
Protein Scaffold Information of This SBP
Scaffold ID PS006
Scaffold Info
[1]
Scaffold Name Affimer
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets+ Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Yeast small ubiquitin-related modifier
BTS Info
Binder Tools for molecular recognition Kd: 33.3 nM University of Leeds [1]
References
1 Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein Eng Des Sel. 2014 May;27(5):145-55.