Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS030)
PSS Name
Designed TPR protein
Synonyms
Designed tetratricopeptide repeat; Consensus-designed tetratricopeptide repeat; CTPR
Diagrammatic Sketch
Highest Status Research
Class Non-Antibody
Fold Type Alpha-Helices + Beta-Turns
Thermal Denaturation TEMP 48-72℃
Sequence Length 18+34 * n
Molecular Weight 11-12 kDa
Parental Protein of This PSS
Parental Protein Name Synonym PDB ID UniProt ID Origin
Tetratricopeptide repeat TPR1 domain of HOP; TPR2A domain of HOP
1ELW
Q86TV6
Human; Synthetic construct
Synthetic Binding Protein (SBP) Featuring this PSS
SBP Name Highest Status Template Expression System Selection Method Details Ref
Designed TPR protein anti-Dss1 T-Mod(Dss1A) Research TPR2A domain Escherichia coli N.A.
SBP Info
 		[1]
Designed TPR protein anti-Dss1 T-Mod(Dss1B) Research TPR2A domain Escherichia coli N.A.
SBP Info
 		[1]
Designed TPR protein anti-Dss1 T-Mods Research TPR2A domain Escherichia coli N.A.
SBP Info
 		[1]
Designed TPR protein anti-hsp90 CTPR390 Research TPR motif Escherichia coli BL21 (DE3) N.A.
SBP Info
 		[2]
Designed TPR protein anti-hsp90 D334K mutant Research TPR2A domain Escherichia coli BL21 (DE3) N.A.
SBP Info
 		[3]
Designed TPR protein anti-HSP90/Hsp70 D334K/T332R double mutant Research TPR2A domain Escherichia coli BL21 (DE3) N.A.
SBP Info
 		[3]
Designed TPR protein anti-PTIEEVD clone TPR1C7 Research TPR1 domain Escherichia coli BL21 (DE3) N.A.
SBP Info
 		[4]
Designed TPR protein anti-hsp90 CTPR390+ Research CTPR3 N.A. N.A.
SBP Info
 		[5]
Designed TPR protein anti-Keap1 Charge Nrf2 CTPR2 Research N.A. Escherichia coli N.A.
SBP Info
 		[6]
Designed TPR protein anti-Keap1 CIDER Nrf2 CTPR2 Research N.A. Escherichia coli N.A.
SBP Info
 		[6]
Designed TPR protein anti-Keap1 Flexible Nrf2 CTPR2 Research N.A. Escherichia coli N.A.
SBP Info
 		[6]
References
1 Designed proteins to modulate cellular networks. ACS Chem Biol. 2010 Jun 18;5(6):545-52.
2 Protein design to understand peptide ligand recognition by tetratricopeptide repeat proteins. Protein Eng Des Sel. 2004 Apr;17(4):399-409.
3 Electrostatic interactions of Hsp-organizing protein tetratricopeptide domains with Hsp70 and Hsp90: computational analysis and protein engineering. J Biol Chem. 2009 Sep 11;284(37):25364-74.
4 Creating novel proteins by combining design and selection. Protein Eng Des Sel. 2010 Jun;23(6):449-55.
5 Designed TPR modules as novel anticancer agents. ACS Chem Biol. 2008 Mar 20;3(3):161-6.
6 Exploring new strategies for grafting binding peptides onto protein loops using a consensus-designed tetratricopeptide repeat scaffold. Protein Sci. 2019 Apr;28(4):738-745.