Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000779)
SBP Name
Designed TPR protein anti-HSP90/Hsp70 D334K/T332R double mutant
Synonyms
Designed TPR protein Hsp70 D334K/T332R double mutant
Thermal Denaturation TEMP 48 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Highest Status Research
Template Name TPR2A domain
Protein Scaffold Information of This SBP
Scaffold ID PS030
Scaffold Info
[1]
Scaffold Name Designed TPR protein
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Beta-Turns
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Heat shock protein HSP 90-beta
BTS Info
Binder Cancers [ICD-11: 2D4Z] Kd: 60 nM Yale University [1]
Heat shock 70 kDa protein 1A
BTS Info
Binder Cancers [ICD-11: 2D4Z] Kd: 3200 nM Yale University [1]
References
1 Electrostatic interactions of Hsp-organizing protein tetratricopeptide domains with Hsp70 and Hsp90: computational analysis and protein engineering. J Biol Chem. 2009 Sep 11;284(37):25364-74.