Details of the PSS | SYNBIP

General Information of Protein Scaffold of SBP (PSS) (ID: PS043)
PSS Name	Kunitz domain
Synonyms	Kunitz domain-based binder
Diagrammatic Sketch
Highest Status	Marketed
Class	Non-Antibody
Fold Type	Alpha-Helices + Beta-Sheets + Loops
Thermal Denaturation TEMP	95℃
Sequence Length	56-58
Molecular Weight	7-8 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym		PDB ID	UniProt ID	Origin
Alzheimer's amyloid beta-protein precursor inhibitor	APPI		1AAP	P05067	Human
Beef pancreas trypsin inhibitor	BPTI		1ZR0	P00974	Bovine
Human lipoprotein-associated coagulation inhibitor domain 1	LACI-D1		4BQD	Q59EE5	Human
Inter-a-trypsin inhibitor	ITI-D2		N.A.	N.A.	N.A.
Leech-derived trypsin inhibitor	LDTI		1LDT	P80424	Leech
Mustard trypsin inhibitor II	MTI II		1JXC	P38057	Sinapis
Pancreatic secretory trypsin inhibitor	Serine protease inhibitor Kazal-type 1; Tumor-associated trypsin inhibitor; TATI; PSTI		1CGI ; 1CGJ ; 1HPT	P00995	Human; Synthetic construct
Periplasmic E. coli protease inhibitor Ecotin	Ecotin		1EZU	P23827	Escherichia coli

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
Kunitz domain Ecallantide	Marketed	N.A.	N.A.	N.A.	SBP Info	[1], [2]
Kunitz domain Depelstat	Phase II	BPTI	N.A.	N.A.	SBP Info	[1], [3]
Kunitz domain anti-PKK EPI-K503	Research	LACI-D1	Saccharomyces cerevisiae	Phage display	SBP Info	[4]
Kunitz domain anti-PKK/FXIa KALI-DY	Research	APPI	Escherichia coli 27C7	Phage display	SBP Info	[5]
Kunitz domain anti-plasmin EPI-P211	Research	LACI-D1	Pichia pastoris	Phage display	SBP Info	[6], [7]
Kunitz domain anti-plasmin EPI-P302	Research	LACI-D1	Pichia pastoris	Phage display	SBP Info	[6], [7]
Kunitz domain anti-FVIIa variant	Research	N.A.	Escherichia coli	Phage display	SBP Info	[8]
Kunitz domain anti-plasmin DX-1000	Research	LACD-D1	N.A.	N.A.	SBP Info	[9]

References

1	In vitro-engineered non-antibody protein therapeutics. Protein Cell. 2018 Jan;9(1):3-14.
2	KEGG DRUG Database. Ecallantide
3	Non-immunoglobulin scaffolds: a focus on their targets. Trends Biotechnol. 2015 Jul;33(7):408-18.
4	Iterative optimization of high-affinity protease inhibitors using phage display. 2. Plasma kallikrein and thrombin. Biochemistry. 1996 Jun 18;35(24):8058-67.
5	Potent and selective Kunitz domain inhibitors of plasma kallikrein designed by phage display. J Biol Chem. 1995 Oct 27;270(43):25411-7.
6	Engineered protein scaffolds for molecular recognition. J Mol Recognit. Jul-Aug 2000;13(4):167-87.
7	Iterative optimization of high-affinity proteases inhibitors using phage display. 1. Plasmin. Biochemistry. 1996 Jun 18;35(24):8045-57.
8	Kunitz domain inhibitors of tissue factor-factor VIIa. II. Potent and specific inhibitors by competitive phage selection. J Biol Chem. 1994 Sep 2;269(35):22137-44.
9	PEGylated DX-1000: pharmacokinetics and antineoplastic activity of a specific plasmin inhibitor. Neoplasia. 2007 Nov;9(11):927-37.