Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP000422)
SBP Name
Kunitz domain anti-plasmin EPI-P302
Synonyms
Kunitz domain EPI-P302
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Pichia pastoris
Selection Method Phage display
Highest Status Research
Sequence Length 58
Template Name LACI-D1
Protein Scaffold Information of This SBP
Scaffold ID PS043
Scaffold Info
[1] , [2]
Scaffold Name Kunitz domain
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Plasmin
BTS Info
Inhibitor Alzheimer disease [ICD-11: 8A20] Kd: 0.087 nM Technical University of Munich [1] , [2]
References
1 Engineered protein scaffolds for molecular recognition. J Mol Recognit. Jul-Aug 2000;13(4):167-87.
2 Iterative optimization of high-affinity proteases inhibitors using phage display. 1. Plasmin. Biochemistry. 1996 Jun 18;35(24):8045-57.