Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS040)
PSS Name
I-body
Synonyms
NCAM1-based binder
Diagrammatic Sketch
Highest Status Research
Class Non-Antibody
Fold Type Beta-Sheets + Loops
Thermal Denaturation TEMP 20-80℃
Sequence Length 103
Molecular Weight 11-27 kDa
Parental Protein of This PSS
Parental Protein Name Synonym PDB ID UniProt ID Origin
Neural cell adhesion molecule 1 N-CAM-1; NCAM-1
2VKX
P13591 ; P13595
Human; Mouse
Synthetic Binding Protein (SBP) Featuring this PSS
SBP Name Highest Status Template Expression System Selection Method Details Ref
I-body AD-214 Phase I N.A. Escherichia coli N.A.
SBP Info
 		[1]
I-body anti-CXCR4 AD-114 Research N.A. N.A. N.A.
SBP Info
 		[2]
I-body anti-CXCR4 ADCX-99 Research Neural cell adhesion molecule (NCAM) Human embryonic kidney?cells 293 Surface plasmon resonance
SBP Info
 		[3]
I-body anti-CXCR4 AM1-126 Research Neural cell adhesion molecule (NCAM) Human embryonic kidney?cells 293 Surface plasmon resonance
SBP Info
 		[3]
I-body anti-CXCR4 AM1-320 Research Neural cell adhesion molecule (NCAM) Human embryonic kidney?cells 293 Surface plasmon resonance
SBP Info
 		[3]
I-body anti-CXCR4 AM3-114 Research Neural cell adhesion molecule (NCAM) Human embryonic kidney?cells 293 Surface plasmon resonance
SBP Info
 		[3]
I-body anti-CXCR4 AM3-523 Research Neural cell adhesion molecule (NCAM) Human embryonic kidney?cells 293 Surface plasmon resonance
SBP Info
 		[3]
I-body anti-CXCR4 AM4-1121 Research Neural cell adhesion molecule (NCAM) Human embryonic kidney?cells 293 Surface plasmon resonance
SBP Info
 		[3]
I-body anti-CXCR4 AM4-272 Research Neural cell adhesion molecule (NCAM) Human embryonic kidney?cells 293 Surface plasmon resonance
SBP Info
 		[3]
I-body anti-CXCR4 AM4-746 Research Neural cell adhesion molecule (NCAM) Human embryonic kidney?cells 293 Surface plasmon resonance
SBP Info
 		[3]
I-body anti-RANKL ADR3 Research N.A. N.A. N.A.
SBP Info
 		[4]
I-body anti-CXCR4 AD-114 Research N.A. Escherichia coli; Pichia pastoris N.A.
SBP Info
 		[5], [1]
I-body anti-CXCR4 AD-114-Im7-FH Research N.A. Escherichia coli N.A.
SBP Info
 		[6]
I-body anti-CXCR4 AD-114-Im7-FH-PEG (2 x 20K) Research N.A. Escherichia coli N.A.
SBP Info
 		[6]
I-body anti-CXCR4 AD-114-Im7-FH-PEG (30K) Research N.A. Escherichia coli N.A.
SBP Info
 		[6]
I-body anti-CXCR4 AD-114-PA600 Research N.A. Escherichia coli N.A.
SBP Info
 		[6]
I-body anti-CXCR4 AD-114-PA600-6H Research N.A. Escherichia coli N.A.
SBP Info
 		[6]
I-body anti-CXCR4/HSA AD-114-Im7-FH-SA21 Research N.A. Escherichia coli N.A.
SBP Info
 		[6]
References
1 AdAlta. Product Development Pipeline. 2021.
2 A single-domain i-body, AD-114, attenuates renal fibrosis through blockade of CXCR4. JCI Insight. 2022 Feb 22;7(4):e143018.
3 i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4. J Biol Chem. 2016 Jun 10;291(24):12641-12657.
4 ADR3, a next generation i-body to human RANKL, inhibits osteoclast formation and bone resorption. J Biol Chem. 2023 Feb;299(2):102889.
5 Anti-fibrotic Effects of CXCR4-Targeting i-body AD-114 in Preclinical Models of Pulmonary Fibrosis. Sci Rep. 2018 Feb 16;8(1):3212.
6 Half-life extension and non-human primate pharmacokinetic safety studies of i-body AD-114 targeting human CXCR4. MAbs. 2019 Oct;11(7):1331-1340.