Details of the PSS | SYNBIP

General Information of Protein Scaffold of SBP (PSS) (ID: PS049)
PSS Name	Neocarzinostatin-based binder
Diagrammatic Sketch
Highest Status	Research
Class	Non-Antibody
Fold Type	Beta-Sheets + Loops
Thermal Denaturation TEMP	51-57℃
Sequence Length	112-132
Molecular Weight	10-14 kDa

Parental Protein of This PSS

Parental Protein Name	Synonym		PDB ID	UniProt ID	Origin
Neocarzinostatin	NCS		1NOA	P0A3R9	Streptomyces; Synthetic construct

Synthetic Binding Protein (SBP) Featuring this PSS

SBP Name	Highest Status	Template	Expression System	Selection Method	Details	Ref
Neocarzinostatin-based binder anti-EtdBr C5-32	Research	Apo-NCS	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[1]
Neocarzinostatin-based binder anti-EtdBr C5-37	Research	Apo-NCS	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[1]
Neocarzinostatin-based binder anti-EtdBr C5-38	Research	Apo-NCS	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[1]
Neocarzinostatin-based binder anti-EtdBr C5-54	Research	Apo-NCS	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[1]
Neocarzinostatin-based binder anti-EtdBr S98A mutant	Research	Apo-NCS	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[1]
Neocarzinostatin-based binder anti-EtdBr S98C mutant	Research	Apo-NCS	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[1]
Neocarzinostatin-based binder anti-EtdBr S98G mutant	Research	Apo-NCS	Escherichia coli BL21 (DE3)	Phage display	SBP Info	[1]
Neocarzinostatin-based binder anti-Testosterone NCS-1a.15	Research	Neocarzinostatin	Escherichia coli	Phage display	SBP Info	[2], [3]
Neocarzinostatin-based binder anti-Testosterone NCS-1b.6	Research	Neocarzinostatin	Escherichia coli	Phage display	SBP Info	[2], [3]
Neocarzinostatin-based binder anti-Testosterone NCS-3.24	Research	Neocarzinostatin	Escherichia coli	Phage display	SBP Info	[2], [3]
Neocarzinostatin-based binder anti-Testosterone NCS-4.1	Research	Neocarzinostatin	Escherichia coli	Phage display	SBP Info	[2], [3]
Neocarzinostatin-based binder anti-Testosterone NCS-4.4	Research	Neocarzinostatin	Escherichia coli	Phage display	SBP Info	[2], [3]
Neocarzinostatin-based binder anti-Testosterone NCS-A6	Research	NCS-3.24	Escherichia coli	Phage display	SBP Info	[4]
Neocarzinostatin-based binder anti-Testosterone NCS-C7	Research	NCS-3.24	Escherichia coli	Phage display	SBP Info	[4]
Neocarzinostatin-based binder anti-Testosterone NCS-C8	Research	NCS-3.24	Escherichia coli	Phage display	SBP Info	[4]
Neocarzinostatin-based binder anti-Testosterone NCS-E8	Research	NCS-3.24	Escherichia coli	Phage display	SBP Info	[4]
Neocarzinostatin-based binder anti-HEWL NCS-CDR3	Research	Neocarzinostatin	Escherichia coli BL21 (DE3)	N.A.	SBP Info	[5]

References

1	Preparation of neocarzinostatin apoprotein mutants and the randomized library on the chromophore-binding cavity. Biol Pharm Bull. 2006 May;29(5):1010-4.
2	Structures of in vitro evolved binding sites on neocarzinostatin scaffold reveal unanticipated evolutionary pathways. J Mol Biol. 2006 Apr 28;358(2):455-71.
3	In vitro evolution of the binding specificity of neocarzinostatin, an enediyne-binding chromoprotein. Biochemistry. 2003 May 20;42(19):5674-83.
4	Disulfide bond substitution by directed evolution in an engineered binding protein. Chembiochem. 2009 May 25;10(8):1349-59.
5	Affinity transfer by CDR grafting on a nonimmunoglobulin scaffold. Protein Sci. 2004 Jul;13(7):1882-91.