General Information of Synthetic Binding Protein (SBP) (ID: SBP000752)
SBP Name
Neocarzinostatin-based binder anti-EtdBr S98C mutant
Synonyms
Neocarzinostatin-based binder S98C mutant
Molecular Weight 11.1 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Phage display
Highest Status Research
Sequence Length 113
SBP Sequence
>Neocarzinostatin-based binder anti-EtdBr S98C mutant
AAPTATVTPSSGLSDGTVVKVAGAGLQAGTAYDVGQCAWVDTGVLACNPADFSSVTADAN
GSASTSLTVRRSFEGFLFDGTRWGTVDCTTAACQVGLCDAAGNGPEGVAISFN
3D Structure
Computationally Modelled Structure
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Template Name Apo-NCS
Protein Scaffold Information of This SBP
Scaffold ID PS049
Scaffold Info
[1]
Scaffold Name Neocarzinostatin-based binder
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Ethidium bromide
BTS Info
Binder Tools for increasing calculated maximum change of the total fluorescence intensity Kd: 9700 nM Tohoku University [1]
References
1 Preparation of neocarzinostatin apoprotein mutants and the randomized library on the chromophore-binding cavity. Biol Pharm Bull. 2006 May;29(5):1010-4.