Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS024)
PSS Name
Cytochrome b562-based binder
Diagrammatic Sketch
Highest Status Research
Class Non-Antibody
Fold Type Alpha-Helices + Loops
Thermal Denaturation TEMP 61-65℃
Sequence Length 106
Molecular Weight 11-13 kDa
Parental Protein of This PSS
Parental Protein Name Synonym PDB ID UniProt ID Origin
Cytochrome b562 Cytochrome b(562); c-b562
256B
P0ABE7 ; P16670
Bacteria
Synthetic Binding Protein (SBP) Featuring this PSS
SBP Name Highest Status Template Expression System Selection Method Details Ref
Cytochrome b562-based binder anti-BSA-1 conjugate C13 Research Cytochrome b562 Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
Cytochrome b562-based binder anti-BSA-1 conjugate C18 Research Cytochrome b562 Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
Cytochrome b562-based binder anti-BSA-1 conjugate C22 Research Cytochrome b562 Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
Cytochrome b562-based binder anti-BSA-1 conjugate C30 Research Cytochrome b562 Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
Cytochrome b562-based binder anti-Porphyrin M7A variant Research Cytochrome b562 Escherichia coli BL21 (DE3) N.A.
SBP Info
 		[2]
Cytochrome b562-based binder anti-Porphyrin M7D variant Research Cytochrome b562 Escherichia coli BL21 (DE3) N.A.
SBP Info
 		[2]
Cytochrome b562-based binder anti-Porphyrin M7E variant Research Cytochrome b562 Escherichia coli BL21 (DE3) N.A.
SBP Info
 		[2]
Cytochrome b562-based binder anti-Zn-Ce6 apo-I17C mutant Research H63N mutant of Cytochrome b562 Escherichia coli N.A.
SBP Info
 		[3]
Cytochrome b562-based binder anti-Heme F61I Variant Research N.A. Escherichia coli N.A.
SBP Info
 		[4]
Cytochrome b562-based binder anti-Heme F65Y Variant Research N.A. Escherichia coli N.A.
SBP Info
 		[4]
References
1 Alternate protein frameworks for molecular recognition. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6552-6.
2 Reengineering cyt b562 for hydrogen production: A facile route to artificial hydrogenases. Biochim Biophys Acta. 2016 May;1857(5):598-603.
3 Protein engineering of cytochrome b562 for quinone binding and light-induced electron transfer. Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17675-80.
4 Cytochrome b562 variants: a library for examining redox potential evolution. Biochemistry. 2000 May 23;39(20):6075-82.