General Information of Synthetic Binding Protein (SBP) (ID: SBP000875)
SBP Name
Cytochrome b562-based binder anti-Porphyrin M7D variant
Synonyms
Cytochrome b562-based binder M7D variant
Molecular Weight 11.8 kDa
Thermal Denaturation TEMP 64.8 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Highest Status Research
Sequence Length 106
SBP Sequence
>Cytochrome b562-based binder anti-Porphyrin M7D variant
ADLEDNDETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKD
FRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR
3D Structure
Computationally Modelled Structure
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Template Name Cytochrome b562
Protein Scaffold Information of This SBP
Scaffold ID PS024
Scaffold Info
[1]
Scaffold Name Cytochrome b562-based binder
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Porphyrin
BTS Info
Binder Tools as a promising avenue to sustainable hydrogen production Kd: 3500 nM Arizona State University [1]
References
1 Reengineering cyt b562 for hydrogen production: A facile route to artificial hydrogenases. Biochim Biophys Acta. 2016 May;1857(5):598-603.