General Information of Synthetic Binding Protein (SBP) (ID: SBP003458)
SBP Name
Nanobody anti-Gs Nb35
Synonyms
Nanobody-35; Nanobody 35; Nb35
Molecular Weight 13.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli WK6
Selection Method Phage display
Highest Status Research
PDB ID 6NBF; 6NBH; 6NBI; 6PB0; 6PB1; 6VN7; 7CFM; 7CFN; 7CX2; 7CX3; 7CX4; 7CZ5; 7D68; 7DTY; 7DUQ; 7E14; 7EVM; 7JV5; 7JVP; 7JVQ; 7DUR
Sequence Length 128
SBP Sequence
>Nanobody anti-Gs Nb35
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSS
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1] , [2]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
BTS Info
Binder Research tool N.A. Stanford University School of Medicine [1] , [2]
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
BTS Info
Binder Research tool N.A. Stanford University School of Medicine [1] , [2]
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
BTS Info
Binder Research tool N.A. Stanford University School of Medicine [1] , [2]
References
1 Structural insights into the human D1 and D2 dopamine receptor signaling complexes. Cell. 2021 Feb 18;184(4):931-942.e18.
2 Crystal structure of the 2 adrenergic receptor-Gs protein complex. Nature. 2011 Jul 19;477(7366):549-55.