Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001013)
SBP Name	Diabody anti-VEGF x anti-PD-1
Synonyms	Anti-VEGF x anti-PD-1 diabody
Molecular Weight	51 kDa
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Pichia pastoris GS115
Highest Status	Research
Template Name	Human VEGF165 mAb and Nivolumab

Protein Scaffold Information of This SBP
Scaffold ID	PS031 Scaffold Info					[1] , [2]
Scaffold Name	Diabody
Scaffold Class	Antibody fragment
Fold Type	Beta-Sheets + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
Vascular endothelial growth factor 165	BTS Info	Inhibitor	Tumors [ICD-11: XH1N44]	Kd: 124.9 nM	China Pharmaceutical University	[1] , [2]
Programmed cell death protein 1	BTS Info	Inhibitor	Tumors [ICD-11: XH1N44]	Kd: 25.1 nM	China Pharmaceutical University	[1] , [2]

References

1	Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in Pichia pastoris and Evaluation of Antitumor Activity In Vivo. Int J Mol Sci. 2018 Sep 25;19(10):2900.
2	Isolation and characterization of human anti-VEGF165 monoclonal antibody with anti-tumor efficacy from transgenic mice expressing human immunoglobulin loci. Cancer Lett. 2009 Jan 8;273(1):28-34.