Details of the PSS

General Information of Protein Scaffold of SBP (PSS) (ID: PS061)
PSS Name
TEM1-beta-lactamase based binder
Diagrammatic Sketch
Highest Status Research
Class Non-Antibody
Fold Type Alpha-Helices + Beta-Sheets + Loops
Thermal Denaturation TEMP 50-56℃
Sequence Length 265-290
Molecular Weight 30-32 kDa
Parental Protein of This PSS
Parental Protein Name Synonym PDB ID UniProt ID Origin
TEM1-beta-lactamase Beta-lactamase TEM
4RVA
P62593
Bacteria; Synthetic construct
Synthetic Binding Protein (SBP) Featuring this PSS
SBP Name Highest Status Template Expression System Selection Method Details Ref
TEM1-beta-lactamase based binder anti-Beta-galactosidase clone 601 Research FdBla Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
TEM1-beta-lactamase based binder anti-Beta-galactosidase clone 604 Research FdBla Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
TEM1-beta-lactamase based binder anti-Ferritin clone 503-1 Research Fer503 Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
TEM1-beta-lactamase based binder anti-Ferritin clone 503-3 Research Fer503 Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
TEM1-beta-lactamase based binder anti-mAbs P10L4-01 Research FdBla Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[2]
TEM1-beta-lactamase based binder anti-mAbs P19L3-01 Research FdBla Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[2]
TEM1-beta-lactamase based binder anti-mAbs P19L4-01 Research FdBla Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[2]
TEM1-beta-lactamase based binder anti-mAbs P66L4-05 Research FdBla Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[2]
TEM1-beta-lactamase based binder anti-SV SVL1-01 Research FdBla Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
TEM1-beta-lactamase based binder anti-SV SVL3-02 Research FdBla Escherichia coli BL21 (DE3) Phage display
SBP Info
 		[1]
TEM1-beta-lactamase based binder anti-BLIP E110G/E239G mutant Research TEM1 N.A. Yeast display
SBP Info
 		[3]
TEM1-beta-lactamase based binder anti-BLIP K51R/E110G mutant Research TEM1 N.A. Yeast display
SBP Info
 		[3]
TEM1-beta-lactamase based binder anti-BLIP V30Q/H112R/T128S/E171K/E197K mutant Research TEM1 N.A. Yeast display
SBP Info
 		[3]
References
1 TEM-1 beta-lactamase as a scaffold for protein recognition and assay. Protein Sci. 2002 Jun;11(6):1506-18.
2 Engineering a regulatable enzyme for homogeneous immunoassays. Nat Biotechnol. 1999 Jan;17(1):67-72.
3 Selecting for Fast Protein-Protein Association As Demonstrated on a Random TEM1 Yeast Library Binding BLIP. Biochemistry. 2018 Aug 7;57(31):4644-4650.