General Information of Synthetic Binding Protein (SBP) (ID: SBP000858)
SBP Name
TEM1-beta-lactamase based binder anti-BLIP E110G/E239G mutant
Synonyms
TEM1-beta-lactamase based binder E110G/E239G mutant
Thermal Denaturation TEMP 55 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Selection Method Yeast display
Highest Status Research
Template Name TEM1
Protein Scaffold Information of This SBP
Scaffold ID PS061
Scaffold Info
[1]
Scaffold Name TEM1-beta-lactamase based binder
Scaffold Class Non-Antibody
Fold Type Alpha-Helices + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Beta-lactamase inhibitory protein
BTS Info
Binder Research tool Kd: 0.18 nM Weizmann Institute of Science [1]
References
1 Selecting for Fast Protein-Protein Association As Demonstrated on a Random TEM1 Yeast Library Binding BLIP. Biochemistry. 2018 Aug 7;57(31):4644-4650.