General Information of Synthetic Binding Protein (SBP) (ID: SBP003425)
SBP Name
CI2-based binder anti-Subtilisin-BPN M59R/E60S mutant
Synonyms
CI2-based binder M59R/E60S mutant
Molecular Weight 7.3 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Highest Status Research
PDB ID 1Y4A; 1Y4D
Sequence Length 64
SBP Sequence
>CI2-based binder anti-Subtilisin-BPN M59R/E60S mutant
MKTEWPELVGKSVEEAKKVILQDKPAAQIIVLPVGTIVTRSYRIDRVRLFVDRLDNIAQV
PRVG
3D Structure
Experimentally Validated Structure
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Template Name CI2
Protein Scaffold Information of This SBP
Scaffold ID PS022
Scaffold Info
[1]
Scaffold Name CI2-based binder
Scaffold Class Non-Antibody
Fold Type One Alpha-Helix + Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Subtilisin BPN'
BTS Info
Inhibitor Research tool Kd: 1.2 nM University of California [1]
References
1 Role of the intramolecular hydrogen bond network in the inhibitory power of chymotrypsin inhibitor 2. Biochemistry. 2005 May 10;44(18):6823-30.