Details of the BTS
| General Information of Binding Target of SBP (BTS) (ID: ST00348) | ||||||
|---|---|---|---|---|---|---|
| BTS Name |
Subtilisin BPN'
|
|||||
| Synonyms |
EC 3.4.21.62; Alkaline protease; Subtilisin DFE; Subtilisin Novo
|
|||||
| BTS Type |
Protein
|
|||||
| Family |
Peptidase S8 family
|
|||||
| Gene Name |
apr
|
|||||
| Organism |
Bacillus amyloliquefaciens (Bacillus velezensis)
|
|||||
| Function |
Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.
|
|||||
| UniProt ID | ||||||
| UniProt Entry | ||||||
| PFam | ||||||
| Sequence |
MRGKKVWISLLFALALIFTMAFGSTSSAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVI
SEKGGKVQKQFKYVDAASATLNEKAVKELKKDPSVAYVEEDHVAHAYAQSVPYGVSQIKA PALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETNPFQDNNSHGTHVAGTVA ALNNSIGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSA ALKAAVDKAVASGVVVVAAAGNEGTSGSSSTVGYPGKYPSVIAVGAVDSSNQRASFSSVG PELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENT TTKLGDSFYYGKGLINVQAAAQ |
|||||
| Sequence Length |
382
|
|||||
| Synthetic Binding Protein (SBP) Targeting This BTS |
|---|
| SBP Name | Highest Status | Mechanism | Affinity | Application | Details | Ref |
|---|---|---|---|---|---|---|
| CI2-based binder anti-Subtilisin-BPN E60A mutant | Research | Inhibitor | Kd: 0.52 nM | Research tool | [1] | |
| CI2-based binder anti-Subtilisin-BPN E60S mutant | Research | Inhibitor | Kd: 0.31 nM | Research tool | [1] | |
| CI2-based binder anti-Subtilisin-BPN M59R/E60S mutant | Research | Inhibitor | Kd: 1.2 nM | Research tool | [1] | |
| CI2-based binder anti-Subtilisin-BPN R62A mutant | Research | Inhibitor | Kd: 0.025 nM | Research tool | [1] | |
| CI2-based binder anti-Subtilisin-BPN R65A mutant | Research | Inhibitor | Kd: 1.7 nM | Research tool | [1] | |
| CI2-based binder anti-Subtilisin-BPN R67A mutant | Research | Inhibitor | Kd: 0.56 nM | Research tool | [1] | |
| CI2-based binder anti-Subtilisin-BPN T58A mutant | Research | Inhibitor | Kd: 0.3 nM | Research tool | [1] | |
| CI2-based binder anti-Subtilisin-BPN T58P mutant | Research | Inhibitor | Kd: 1.7 nM | Research tool | [1] | |