Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001529)
SBP Name
Fab anti-Digitoxin/Gitoxin 26-10
Synonyms
Fab Gitoxin 26-10
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Selection Method Phage display
Highest Status Research
PDB ID 1IGJ; 1IGI
SBP Sequence
>Heavy Chain
EVQLQQSGPELVKPGASVRMSCKSSGYIFTDFYMNWVRQSHGKSLDYIGYISPYSGVTGY
NQKFKGKATLTVDKSSSTAYMELRSLTSEDSAVYYCAGSSGNKWAMDYWGHGASVTVSSA
KTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDL
YTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEP
>Light Chain
DVVMTQTPLSLPVSLGDQASISCRSSQSLVHSNGNTYLNWYLQKAGQSPKLLIYKVSNRF
SGVPDRFSGSGSGTDFTLKISRVEAEDLGIYFCSQTTHVPPTFGGGTKLEIKRADAAPTV
SIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSM
SSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS034
Scaffold Info
[1] , [2]
Scaffold Name Fab
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Gitoxin
BTS Info
Binder Tools for modifying specificity of antidigoxin antibodies N.A. Massachusetts General Hospital and Harvard Medical School; Massachusetts General Hospital [1] , [2]
Digitoxin
BTS Info
Binder Tools for modifying specificity of antidigoxin antibodies N.A. Massachusetts General Hospital and Harvard Medical School; Massachusetts General Hospital [1] , [2]
References
1 Complementary combining site contact residue mutations of the anti-digoxin Fab 26-10 permit high affinity wild-type binding. J Biol Chem. 2002 May 10;277(19):16365-70.
2 A single H:CDR3 residue in the anti-digoxin antibody 26-10 modulates specificity for C16-substituted digoxin analogs. Protein Eng. 2001 Apr;14(4):287-96.