General Information of Synthetic Binding Protein (SBP) (ID: SBP001025)
SBP Name
Diabody anti-MBP 6683
Synonyms
Diabody 6683
Molecular Weight 54.1 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System High Five insect cells
Highest Status Research
Sequence Length 504
SBP Sequence
>Diabody anti-MBP 6683
QVQLKESGPGLVRPSQSLSLTCSVTGYSITSGYYWNWIRQFPGNKLEWMGYISYDGSNNY
NPSLKGRISITRDTSKNQFFLKLNSVTTDDTATYYCARAYIGFAYWGQGTLVTVSSGGGG
SDIVLTQPPSVSVAPGQTARISCSGDNIGSYYVHWYQQKPGQAPVLVIYEDSERPSGIPE
RFSGSNSGNTATLTISGTQAEDEADYYCSSYDDPNFQVFGGGTKLTVKPWLVPRGSHHHH
HHSGGGGSGGGGSGGGGSGGGGLVPRGSPGQVQLQQSGPGLVKPSQTLSLTCAISGDSVS
SNSAAWGWIRQSPGRGLEWLGIIQKRSKWYNNYAVKVKSKITINPDTSKNQFSLQLSSVT
PEDTAVYYCARYSYPFYSIDYWGQGTLVTVSSGGGGSDIQLTQSTSSLPASLGDRVTISC
RAGQDISNHLNWYQQKPDGTVKLLIYYTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDI
ATYFCQQGNTLPWTFGGGSKLEIK
3D Structure
Computationally Modelled Structure
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Template Name Diabody (PDBID 1LMK)
Protein Scaffold Information of This SBP
Scaffold ID PS031
Scaffold Info
[1]
Scaffold Name Diabody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Maltose/maltodextrin-binding periplasmic protein
BTS Info
Binder Tools for analyzing cryo-electron microscopic images and building protein nano-assemblies N.A. Graduate School of Nanoscience and Technology [1]
References
1 Crystal structures of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface. Sci Rep. 2016 Sep 29;6:34515.