Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001011)
SBP Name
Diabody anti-CEA T84.66
Synonyms
Diabody T84.66
Molecular Weight 55 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Mammalian cells
Highest Status Research
PDB ID 1MOE
3D Structure
Experimentally Validated Structure
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Template Name Antibody T84.66
Protein Scaffold Information of This SBP
Scaffold ID PS031
Scaffold Info
[1] , [2]
Scaffold Name Diabody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Carcinoembryonic antigen
BTS Info
Binder Tools for rapid tumor targeting N.A. Beckman Research Institute of the City of Hope [1] , [2]
Clinical Trial Information of This SBP
NCT00647153 Click to show the Detail
Indication Colorectal Cancer
Phase Phase I
Title Pre-Surgical Detection of Colorectal Carcinomas Using 123Iodine-Labeled cT84.66 Diabody Antibody Fragments to Carcinoembryonic Antigen (CEA)
Status Completed
Sponsor City of Hope Medical Center
References
1 Mammalian expression and hollow fiber bioreactor production of recombinant anti-CEA diabody and minibody for clinical applications. J Immunol Methods. 2001 Jul 1;253(1-2):195-208.
2 The crystal structure of an anti-CEA scFv diabody assembled from T84.66 scFvs in V(L)-to-V(H) orientation: implications for diabody flexibility. J Mol Biol. 2003 Feb 14;326(2):341-51.