General Information of Synthetic Binding Protein (SBP) (ID: SBP000714)
SBP Name
VNAR anti-EpCAM A2
Synonyms
VNAR A2
Molecular Weight 12 kDa
Thermal Denaturation TEMP 55-59 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Expi293F?human cells
Selection Method Yeast display
Highest Status Research
SBP Sequence
>CDR3
YLKHHAKYVGHI
Protein Scaffold Information of This SBP
Scaffold ID PS065
Scaffold Info
[1]
Scaffold Name vNAR
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Epithelial cell adhesion molecule
BTS Info
Binder Research tool Kd: 349 nM Technical University of Darmstadt [1]
References
1 Isolation of a pH-Sensitive IgNAR Variable Domain from a Yeast-Displayed, Histidine-Doped Master Library. Mar Biotechnol (NY). 2016 Apr;18(2):161-7.