Details of the SBP
| General Information of Synthetic Binding Protein (SBP) (ID: SBP000512) | ||||||
|---|---|---|---|---|---|---|
| SBP Name |
Affibody anti-Raf-1 Z(RAF322: 01)
|
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| Synonyms |
Affibody Z(RAF322: 01)
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| Molecular Weight | 6.5 kDa | |||||
| Thermal Denaturation TEMP | 50.3C51.4 ℃ | |||||
| Design Method | Traditional methods (Site-directed mutagenesis and/or Directed evolution) | |||||
| Expression System | Escherichia coli Rosetta (DE3) | |||||
| Selection Method | Ribosome display | |||||
| Highest Status | Research | |||||
| Sequence Length | 58 | |||||
| SBP Sequence |
>Affibody anti-Raf-1 Z(RAF322
01) |
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| 3D Structure | ||||||
| Computationally Modelled Structure | ||||||
| Click to Save PDB File | ||||||
| Template Name | Z domain of staphylococcal protein A | |||||
| Protein Scaffold Information of This SBP | ||||||
|---|---|---|---|---|---|---|
| Scaffold ID | PS004 | [1] | ||||
| Scaffold Name | Affibody | |||||
| Scaffold Class | Non-Antibody | |||||
| Fold Type | Three Alpha-Helices | |||||
| Binding Target(s) of This SBP (BTS) |
|---|
| BTS Name | Details | Mechanism | Application | Affinity | Research Organization | Ref |
|---|---|---|---|---|---|---|
| RAF proto-oncogene serine/threonine-protein kinase | Inhibitor | Tools for monitoring whole gene in vitro | Kd: 190 nM | Royal Institute of Technology | [1] | |