Details of the BTS
General Information of Binding Target of SBP (BTS) (ID: ST00023) | ||||||
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BTS Name |
RAF proto-oncogene serine/threonine-protein kinase
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Synonyms |
EC 2.7.11.1; Proto-oncogene c-RAF; cRaf; Raf-1
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BTS Type |
Protein
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Family |
Protein kinase superfamily;
TKL Ser/Thr protein kinase family; RAF subfamily |
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Gene Name |
RAF1
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Organism |
Homo sapiens (Human)
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Function |
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
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UniProt ID | ||||||
UniProt Entry | ||||||
PFam | ||||||
Gene ID | ||||||
Sequence |
MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRV
FLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAAS LIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKV PTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTF NTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNL SPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSF GTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIV TQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGL TVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYE LMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFP QILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF |
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Sequence Length |
648
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Synthetic Binding Protein (SBP) Targeting This BTS |
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SBP Name | Highest Status | Mechanism | Affinity | Application | Details | Ref |
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Affibody anti-Raf-1 Z(RAF322) | Research | Inhibitor | Kd: 1900 nM | Tools for monitoring whole gene in vitro | [1] | |
Affibody anti-Raf-1 Z(RAF322: 01) | Research | Inhibitor | Kd: 190 nM | Tools for monitoring whole gene in vitro | [1] | |
Affibody anti-Raf-1 Z(RAF322: 02) | Research | Inhibitor | Kd: 100 nM | Tools for monitoring whole gene in vitro | [1] | |
Affibody anti-Raf-1 Z(RAF322: 03) | Research | Inhibitor | Kd: 120 nM | Tools for monitoring whole gene in vitro | [1] | |
Affibody anti-Raf-1 Z(RAF322: 04) | Research | Inhibitor | Kd: 90 nM | Tools for monitoring whole gene in vitro | [1] | |