General Information of Synthetic Binding Protein (SBP) (ID: SBP000458)
SBP Name
Affibody anti-SasA ZpA963
Synonyms
Affibody ZpA963
Molecular Weight 6.4 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Phage display
Highest Status Research
PDB ID 2M5A
Sequence Length 58
SBP Sequence
>Affibody anti-SasA ZpA963
VDNKFNKETQEASWEIFTLPNLNGRQVAAFISSLLDDPSQSANLLAEAKKLNDAQAPK
3D Structure
Experimentally Validated Structure
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Template Name Z domain of staphylococcal protein A
Protein Scaffold Information of This SBP
Scaffold ID PS004
Scaffold Info
[1]
Scaffold Name Affibody
Scaffold Class Non-Antibody
Fold Type Three Alpha-Helices
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Immunoglobulin G-binding protein A
BTS Info
Binder Research tool Kd: 20 nM Affibody AB [1]
References
1 High-affinity binding to staphylococcal protein A by an engineered dimeric Affibody molecule. Protein Eng Des Sel. 2013 Oct;26(10):635-44.