General Information of Synthetic Binding Protein (SBP) (ID: SBP000457)
SBP Name
Affibody anti-SasA Z(SPA-1)
Synonyms
Affibody Z(SPA-1)
Molecular Weight 6.4 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display
Highest Status Research
PDB ID 1H0T
Sequence Length 58
SBP Sequence
>Affibody anti-SasA Z(SPA-1)
VDNKFNKELSVAGREIVTLPNLNDPQKKAFIFSLWDDPSQSANLLAEAKKLNDAQAPK
3D Structure
Experimentally Validated Structure
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Template Name Z domain of staphylococcal protein A
Protein Scaffold Information of This SBP
Scaffold ID PS004
Scaffold Info
[1]
Scaffold Name Affibody
Scaffold Class Non-Antibody
Fold Type Three Alpha-Helices
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Immunoglobulin G-binding protein A
BTS Info
Binder Tools for expressing anti-idiotypic affibody pair Kd: 2000-6500 nM Royal Institute of Technology [1]
References
1 Anti-idiotypic protein domains selected from protein A-based affibody libraries. Proteins. 2002 Aug 15;48(3):454-62.