General Information of Binding Target of SBP (BTS) (ID: ST00220)
BTS Name
Dipeptide and tripeptide permease A
BTS Type
Protein
Family
Major facilitator superfamily;
Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family;
DtpA subfamily
Gene Name
dtpA
Organism
Escherichia coli (strain K12)
Function
Proton-dependent permease that transports di- and tripeptides as well as structurally related peptidomimetics such as aminocephalosporins into the cell. Has a clear preference for dipeptides and tripeptides composed of L-amino acids, and discriminates dipeptides on the basis of the position of charges within the substrate.
UniProt ID
P77304
UniProt Entry
DTPA_ECOLI
PFam
PF00854
Gene ID
947436
Sequence
MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIMAVYLVKQLGMSEADSI
TLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGYALVAWSGHDAGIVYMGMA
AIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVNIGSFFSMIATPWLAAKYGWS
VAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINYRNLLLTIIGVVALIAIATWLLH
NQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMIVAFILMLEAIIFFVLYSQMPTSLN
FFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSPILAAIYNKMGDTLPMPTKFAIGMVMC
SGAFLILPLGAKFASDAGIVSVSWLVASYGLQSIGELMISGLGLAMVAQLVPQRLMGFIM
GSWFLTTAGANLIGGYVAGMMAVPDNVTDPLMSLEVYGRVFLQIGVATAVIAVLMLLTAP
KLHRMTQDDAADKAAKAAVA
Sequence Length
500
Synthetic Binding Protein (SBP) Targeting This BTS
SBP Name Highest Status Mechanism Affinity Application Details Ref
Nanobody anti-DtpA N00 Research Binder Kd: 59000 nM Research tool
SBP Info
[1]
Nanobody anti-DtpA N21 Research Binder N.A. Research tool
SBP Info
[1]
Nanobody anti-DtpA N87 Research Binder N.A. Research tool
SBP Info
[1]
Nanobody anti-DtpA N89 Research Binder N.A. Research tool
SBP Info
[1]
Nanobody anti-DtpA N93 Research Binder N.A. Research tool
SBP Info
[1]
References
1 Structure of Prototypic Peptide Transporter DtpA from E. coli in Complex with Valganciclovir Provides Insights into Drug Binding of Human PepT1. J Am Chem Soc. 2019 Feb 13;141(6):2404-2412.