General Information of Synthetic Binding Protein (SBP) (ID: SBP001087)
SBP Name
Nanobody anti-DtpA N00
Synonyms
Nanobody N00
Molecular Weight 14.4 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli WK6
Selection Method Phage display
Highest Status Research
PDB ID 6GS7
Sequence Length 132
SBP Sequence
>Nanobody anti-DtpA N00
QVQLQESGGGLVQAGGSLRLSCAGSGRTFSSYNMGWFRQAPGKEREFVGGISWTGRSADY
PDSVKGRFTISRDNAKNAVYLQMNSLKPEDTAVYYCAAKQYGSRADYPWDDYDYWGQGTQ
VTVSSGAAEPEA
3D Structure
Experimentally Validated Structure
Click to Save PDB File
Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Dipeptide and tripeptide permease A
BTS Info
Binder Research tool Kd: 59000 nM Centre for Structural Systems Biology (CSSB) [1]
References
1 Structure of Prototypic Peptide Transporter DtpA from E. coli in Complex with Valganciclovir Provides Insights into Drug Binding of Human PepT1. J Am Chem Soc. 2019 Feb 13;141(6):2404-2412.