Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP003461)
SBP Name
Nanobody anti-C4b hC4Nb8
Synonyms
Nanobody hC4Nb8; hC4Nb8
Molecular Weight 13.6 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Bacteria
Selection Method Phage display
Highest Status Research
PDB ID 6YSQ
Sequence Length 124
SBP Sequence
>Nanobody anti-C4b hC4Nb8
QVQLVESGGGLVQTGDSLRLSCAASGRTFSRYAMGWFRQAPGKERELVAAINWSGGSTYY
ADFAKGRFTISRDNAKNMLYLRMSSLKPEDTAVYYCAAGGPEVEITRANEYDYWGQGTQV
TVSS
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Complement C4-B
BTS Info
Inhibitor Research tool Kd: 0.016 nM Aarhus University [1]
Complement C4-B
BTS Info
Inhibitor Research tool Kd: 0.18 nM Aarhus University [1]
References
1 An Ultrahigh-Affinity Complement C4b-Specific Nanobody Inhibits In Vivo Assembly of the Classical Pathway Proconvertase. J Immunol. 2020 Sep 15;205(6):1678-1694.