General Information of Synthetic Binding Protein (SBP) (ID: SBP003327)
SBP Name
Nanobody anti-GFP cAbGFP4-1-0-1
Synonyms
Nanobody cAbGFP4-1-0-1
Molecular Weight 12.7 kDa
Thermal Denaturation TEMP 75.7 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Highest Status Research
SBP Sequence
>Nanobody anti-GFP SAbGFP4-1-0-1
QVQLVESGGALVQPGGSLRLSSAASGFPVNRYSMRWYRQAPGKEREWVSGMSSAGDRSSY
EDSVKGRFTSSRDDARNTVYLQMNSLKPEDTAVYYSNVNVGFEYWGQGTQVTVSS
3D Structure
Computationally Modelled Structure
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Template Name cAbPSA-N8
Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Green fluorescent protein
BTS Info
Binder Research tool Kd: 0.6 nM Vrije Universiteit Brussel [1]
References
1 Disulfide bond introduction for general stabilization of immunoglobulin heavy-chain variable domains. J Mol Biol. 2008 Mar 21;377(2):478-88.