General Information of Synthetic Binding Protein (SBP) (ID: SBP003308)
SBP Name
Nanobody anti-GFP
Synonyms
Anti-GFP nanobody
Molecular Weight 12.8 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Leishmania tarentolae
Highest Status Research
SBP Sequence
>Nanobody anti-GFP
MQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSS
YEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVSS
3D Structure
Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Green fluorescent protein
BTS Info
Binder Research tool Kd: 0.49 nM European Molecular Biology Laboratory Australia (EMBL Australia) Node in Single Molecule Science [1]
References
1 A Split-Luciferase Reporter Recognizing GFP and mCherry Tags to Facilitate Studies of Protein-Protein Interactions. Int J Mol Sci. 2017 Dec 11;18(12):2681.