General Information of Synthetic Binding Protein (SBP) (ID: SBP003307)
SBP Name
Nanobody anti-SEB D9
Synonyms
Nanobody D9
Molecular Weight 16.3 kDa
Thermal Denaturation TEMP 65 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli Rosetta (DE3)
Selection Method Phage display
Highest Status Research
PDB ID 7KJI
Sequence Length 152
SBP Sequence
>Nanobody anti-SEB D9
LLLAAQPAMAEVQLQASGGGLAQAGDSLRLSCAASIRTFGSYAMGWFRQAPGKEREFVAA
ISWSGGDTYADSAKGRFTISRDNAKNMMYLQMNSLAPEDTAVYFCAIVEAKYYISKDARD
YGYWGQGTQV FVSSEPKTPKPQPAASGAEFAA
3D Structure
Experimentally Validated Structure
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Template Name Consensus sequence
Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Enterotoxin type B
BTS Info
Binder Research tool Kd: 0.11 nM Postdoctoral Fellow at the US Naval Research Laboratory [1]
References
1 Next-Generation Sequencing of a Single Domain Antibody Repertoire Reveals Quality of Phage Display Selected Candidates. PLoS One. 2016 Feb 19;11(2):e0149393.