General Information of Synthetic Binding Protein (SBP) (ID: SBP003297)
SBP Name
Nanobody anti-Ricin D1
Synonyms
sdAb D1
Molecular Weight 12.9 kDa
Thermal Denaturation TEMP 50 ℃
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli Rosetta (DE3)
Selection Method Phage display
Highest Status Research
SBP Sequence
>Nanobody anti-Ricin D1
EVQLQASGGGLVQPGGSLRLACQYSWFRQSPGKEREFVARFTTSSDNFKKTAYLQMNGLK
PEDTALYYCTESQGTQVTVSSGREGTTTVGAIRWTDSRTPHTDSDTWNSVVTGTAADW
3D Structure
Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Ricin
BTS Info
Binder Research tool Kd: 0.5 nM US Naval Research Laboratory [1]
References
1 Contributions of the complementarity determining regions to the thermal stability of a single-domain antibody. PLoS One. 2013 Oct 15;8(10):e77678.