Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP003186)
SBP Name
Nanobody anti-mCherry LaM-2
Synonyms
Nanobody LaM-2
Molecular Weight 14.3 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Phage display
Highest Status Research
PDB ID 6IR2
Sequence Length 131
SBP Sequence
>Nanobody anti-mCherry LaM-2
MAQVQLVESGGGLVQAGGSLRLSCATSGFTFSDYAMGWFRQAPGKEREFVAAISWSGHVT
DYADSVKGRFTISRDNVKNTVYLQMNSLKPEDTAVYSCAAAKSGTWWYQRSENDFGSWGQ
GTQVTVSKEAI
3D Structure
Experimentally Validated Structure
Click to Save PDB File
Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1] , [2]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
MCHERRY
BTS Info
Binder Research tool Kd: 0.49 nM Rockefeller University; European Molecular Biology Laboratory Australia (EMBL Australia) Node in Single Molecule Science [1] , [2]
References
1 A robust pipeline for rapid production of versatile nanobody repertoires. Nat Methods. 2014 Dec;11(12):1253-60.
2 A Split-Luciferase Reporter Recognizing GFP and mCherry Tags to Facilitate Studies of Protein-Protein Interactions. Int J Mol Sci. 2017 Dec 11;18(12):2681.