General Information of Synthetic Binding Protein (SBP) (ID: SBP003136)
SBP Name
Monobody anti-CrSAS-6 clone 13
Synonyms
MB-CRS6-13
Molecular Weight 13.0 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Phage display and yeast display
Highest Status Research
PDB ID 6ZZD
Sequence Length 118
SBP Sequence
>Monobody anti-CrSAS-6 clone 13
SSGLNDIFEAQKIEWHEENLYFQGSVSSVPTKLEVVAATPTSLLISWDAPAVTVYFYVIT
YGETGGNSPVQEFEVPGSKSTATISGLKPGVDYTITVYANNKYSRWYGISPISINYRT
3D Structure
Experimentally Validated Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Centriole protein
BTS Info
Binder Tools for tuning SAS-6 architecture Kd: 134 nM Swiss Federal Institute of Technology Lausanne (EPFL) [1]
References
1 Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly. Nat Commun. 2021 Jun 21;12(1):3805.