General Information of Synthetic Binding Protein (SBP) (ID: SBP003122)
SBP Name
scFv anti-Insulin RD012D5(3)
Synonyms
scFv RD012D5(3)
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Ribosome display
Highest Status Research
SBP Sequence
>VH CDR3
KFGSSLGRADDAFGI
>VL CDR3
RVWDSSTDHWV
Template Name scFv D3
Template Sequence
>VH CDR3
DWGFYGDIWARGFDI
>VL CDR3
QSYDSSLNGHVV
Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Insulin
BTS Info
Binder Research tool Kd: 5.7 nM Cambridge Antibody Technology [1]
References
1 Affinity maturation of phage display antibody populations using ribosome display. J Immunol Methods. 2006 Jun 30;313(1-2):129-39.