General Information of Synthetic Binding Protein (SBP) (ID: SBP003115)
SBP Name
scFv anti-RAGE 3B3
Synonyms
scFv 3B3
Molecular Weight 25.9 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli
Selection Method Phage display and Ribosome display
Highest Status Research
Sequence Length 242
SBP Sequence
>scFv anti-RAGE 3B3
DIQMTQSPSSLSASVGDRVTITCRASQDVGFYVNWFQQKPGKAPRRLIYRATNLADGVPS
RFSGSRSGTDFTLTISSLQPEDFATYYCLEFDEHPLTFGGGTKVEIKDGGGSGGGGSGGG
GSSEVQLVESGGGLVQPGGSLRLSCAASGFTFNNYWMTWVRQAPGKGLEWVASIDNSGDN
TYYPDSVKDRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARGGDITTGIDYWGQGTLVTV
SS
3D Structure
Computationally Modelled Structure
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Template Name scFv M4
Protein Scaffold Information of This SBP
Scaffold ID PS057
Scaffold Info
[1]
Scaffold Name scFv
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Advanced glycosylation end product-specific receptor
BTS Info
Blocker Research tool N.A. University College Dublin [1]
References
1 Affinity maturation of a humanized rat antibody for anti-RAGE therapy: comprehensive mutagenesis reveals a high level of mutational plasticity both inside and outside the complementarity-determining regions. J Mol Biol. 2009 May 8;388(3):541-58.