Details of the SBP | SYNBIP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001855)
SBP Name	DART anti-cadherin/CD3 34
Synonyms	DART CD3 34
Design Method	Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System	Human embryonic kidney?cells 293
Selection Method	Phage display
Highest Status	Research
SBP Sequence	>VH CDR1 SYGIS >VH CDR2 WISAYNGNTNYAQKLQG >VH CDR3 IDTASAFDI >VL CDR1 SGSSSNIGNNYVS >VL CDR2 DNNKRPS >VL CDR3 GTWDSSLSAWV

Protein Scaffold Information of This SBP
Scaffold ID	PS028 Scaffold Info					[1]
Scaffold Name	DART
Scaffold Class	Antibody fragment
Fold Type	Beta-Sheets + Loops

Binding Target(s) of This SBP (BTS)

BTS Name	Details	Mechanism	Application	Affinity	Research Organization	Ref
T-cell surface glycoprotein CD3	BTS Info	Binder	Cancers [ICD-11: 2D4Z]	Kd: 21.9 nM	Pfizer	[1]
Cadherin-3	BTS Info	Binder	Cancers [ICD-11: 2D4Z]	Kd: 129.3 nM	Pfizer	[1]

References

1

Development of PF-06671008, a Highly Potent Anti-P-cadherin/Anti-CD3 Bispecific DART Molecule with Extended Half-Life for the Treatment of Cancer. Antibodies (Basel). 2016 Mar 4;5(1):6.