Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001302)
SBP Name
Monobody anti-GPR56 clone beta12
Synonyms
Monobody mGPR56_beta12
Molecular Weight 10.6 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method Phage display and yeast display
Highest Status Research
Sequence Length 96
SBP Sequence
>Monobody anti-GPR56 clone beta12
VSDVPRDLEVVAATPTSLLISWDAPAVTVDFYFITYGETGWGYGSYQAFEVPGSKSTATI
SGLKPGVDYTITVYAYYYDSQRFLHSGSPISINYRT
3D Structure
Computationally Modelled Structure
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Template Name 10th domain of fibronectin type III (10Fn3)
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1] , [2]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Adhesion G-protein coupled receptor G1
BTS Info
Binder aGPCR-targeted therapeutics Kd: 5.6 nM University of Chicago [1] , [2]
References
1 Specific and direct modulation of the interaction between adhesion GPCR GPR56/ADGRG1 and tissue transglutaminase 2 using synthetic ligands. Sci Rep. 2020 Oct 9;10(1):16912.
2 Stachel-independent modulation of GPR56/ADGRG1 signaling by synthetic ligands directed to its extracellular region. Proc Natl Acad Sci U S A. 2017 Sep 19;114(38):10095-10100.