Details of the SBP

General Information of Synthetic Binding Protein (SBP) (ID: SBP001168)
SBP Name
Monobody anti-Lck-SH2/Hck-SH2 clone 3
Synonyms
Monobody Lck_3
Molecular Weight 10.0 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System K562 Cells; Jurkat cells
Selection Method Phage display, Yeast display and Fluorescence-activated cell sorting
Highest Status Research
PDB ID 5MTM
Sequence Length 93
SBP Sequence
>Monobody anti-Lck-SH2/Hck-SH2 clone 3
VSSVPTKLEVVAATPTSLLISWDAPAVTVLYYLITYGETGDHWSGHQAFEVPGSKSTATI
SGLKPGVDYTITVYAHAESYGESYSPISINYRT
3D Structure
Experimentally Validated Structure
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Template Name Fibronectin type III domain (FN3)
Template Sequence Description X denotes a mixture of 30% Tyr, 15% Ser, 10% Gly, 5% Phe, 5% Trp, and 2.5% each of all the other amino acids except for Cys; O denotes a mixture of Asn, Asp, His, Ile, Leu, Phe, Tyr, and Val; U denotes a mixture of His, Leu, Phe, and Tyr; Z denotes a mixture of Ala, Glu, Lys, and Thr.
Protein Scaffold Information of This SBP
Scaffold ID PS047
Scaffold Info
[1]
Scaffold Name Monobody
Scaffold Class Non-Antibody
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
SrcB subgroup Hck-Src-homology 2
BTS Info
Inhibitor Tools for dissecting SFK functions in normal development and signaling Kd: 364 nM Swiss Institute for Experimental Cancer Research (ISREC) [1]
SrcB subgroup Lck-Src-homology 2
BTS Info
Inhibitor Tools for dissecting SFK functions in normal development and signaling Kd: 19 nM Swiss Institute for Experimental Cancer Research (ISREC) [1]
References
1 Selective Targeting of SH2 Domain-Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies. J Mol Biol. 2017 May 5;429(9):1364-1380.