General Information of Synthetic Binding Protein (SBP) (ID: SBP001160)
SBP Name
Nanobody anti-sbMATE clone 94
Synonyms
Nb94
Molecular Weight 13.4 kDa
Design Method Traditional methods (Site-directed mutagenesis and/or Directed evolution)
Expression System Escherichia coli BL21 (DE3)
Selection Method mRNA display; cDNA display
Highest Status Research
Sequence Length 125
SBP Sequence
>Nanobody anti-sbMATE clone 94
EVQLVESGGGLVQPGGSLRLSCAASGFTFSTYSMSWVRLAPGKALEWVSVINSGGDATYY
ADSVKGRFTISRDNAKNMLYLQMNSLKPVDTAMYYCVTGLYMSTGPVVAVPFGYWGQGTQ
VTVSS
3D Structure
Computationally Modelled Structure
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Protein Scaffold Information of This SBP
Scaffold ID PS048
Scaffold Info
[1]
Scaffold Name Nanobody
Scaffold Class Antibody fragment
Fold Type Beta-Sheets + Loops
Binding Target(s) of This SBP (BTS)
BTS Name Details Mechanism Application Affinity Research Organization Ref
Protein DETOXIFICATION
BTS Info
Modulator Research tool Kd: 99.5 nM University of California at San Diego [1]
References
1 Functional characterization and discovery of modulators of SbMATE, the agronomically important aluminium tolerance transporter from Sorghum bicolor. Sci Rep. 2017 Dec 21;7(1):17996.